Râdegran G, Pilegaard H, Nielsen J J, Bangsbo J
Copenhagen Muscle Research Centre, Rigshospitalet, DK-2200 Copenhagen N, Denmark.
J Appl Physiol (1985). 1998 Aug;85(2):751-7. doi: 10.1152/jappl.1998.85.2.751.
The present study compared the microdialysis ethanol outflow-inflow technique for estimating blood flow (BF) in skeletal muscle of humans with measurements by Doppler ultrasound of femoral artery inflow to the limb (BFFA). The microdialysis probes were inserted in the vastus lateralis muscle and perfused with a Ringer acetate solution containing ethanol, [2-3H]adenosine (Ado), and D-[14C(U)]glucose. BFFA at rest increased from 0.16 +/- 0.02 to 1.80 +/- 0.26 and 4.86 +/- 0.53 l/min with femoral artery infusion of Ado (AdoFA,i) at 125 and 1,000 microg . min-1 . l-1 thigh volume (low dose and high dose, respectively; P < 0.05) and to 3.79 +/- 0.37 and 6.13 +/- 0.65 l/min during one-legged, dynamic, thigh muscle exercise without and with high AdoFA,i, respectively (P < 0.05). The ethanol outflow-to-inflow ratio (38.3 +/- 2.3%) and the probe recoveries (PR) for [2-3H]Ado (35.4 +/- 1.6%) and for D-[14C(U)]glucose (15.9 +/- 1.1%) did not change with AdoFA,i at rest (P = not significant). During exercise without and with AdoFA,i, the ethanol outflow-to-inflow ratio decreased (P < 0.05) to a similar level of 17.5 +/- 3.4 and 20.6 +/- 3.2%, respectively (P = not significant), respectively, while the PR increased (P < 0.05) to a similar level (P = not significant) of 55.8 +/- 2.8 and 61.2 +/- 2. 5% for [2-3H]Ado and to 42.8 +/- 3.9 and 45.2 +/- 5.1% for D-[14C(U)]glucose. Whereas the ethanol outflow-to-inflow ratio and PR correlated inversely and positively, respectively, to the changes in BF during muscular contractions, neither of the ratio nor PR correlated to the AdoFA,i-induced BF increase. Thus the ethanol outflow-to-inflow ratio does not represent skeletal muscle BF but rather contraction-induced changes in molecular transport in the interstitium or over the microdialysis membrane.
本研究将用于评估人体骨骼肌血流（BF）的微透析乙醇流出-流入技术与通过多普勒超声测量股动脉向肢体的流入血流（BFFA）进行了比较。将微透析探针插入股外侧肌，并用含有乙醇、[2-³H]腺苷（Ado）和D-[¹⁴C(U)]葡萄糖的醋酸林格溶液进行灌注。静息时，随着以125和1000μg·min⁻¹·l⁻¹大腿体积（分别为低剂量和高剂量；P<0.05）向股动脉输注Ado（AdoFA,i），BFFA从0.16±0.02增加到1.80±0.26和4.86±0.53l/min，在单腿动态大腿肌肉运动期间，无AdoFA,i和有高剂量AdoFA,i时，BFFA分别增加到3.79±0.37和6.13±0.65l/min（P<0.05）。静息时，乙醇流出与流入比（38.3±2.3%）以及[2-³H]Ado（35.4±1.6%）和D-[¹⁴C(U)]葡萄糖（15.9±1.1%）的探针回收率（PR）不会因AdoFA,i而改变（P=无显著性差异）。在无AdoFA,i和有AdoFA,i的运动期间，乙醇流出与流入比分别降低（P<0.05）至相似水平，分别为17.5±3.4%和20.6±3.2%（P=无显著性差异），而[2-³H]Ado的PR增加（P<0.05）至相似水平（P=无显著性差异），分别为5
