Poulsen L K, Licht T R, Rang C, Krogfelt K A, Molin S
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
J Bacteriol. 1995 Oct;177(20):5840-5. doi: 10.1128/jb.177.20.5840-5845.1995.
Growth rates of Escherichia coli BJ4 colonizing the large intestine of streptomycin-treated mice were estimated by quantitative hybridization with rRNA target probes and by epifluorescence microscopy. The ribosomal contents in bacteria isolated from the cecal mucus, cecal contents, and feces were measured and correlated with the ribosomal contents of bacteria growing in vitro at defined rates. The data suggest that E. coli BJ4 grows at an overall high rate in the intestine. However, when taking into account the total intestinal volume and numbers of bacteria present in cecal mucus, cecal contents, and feces, we suggest that E. coli BJ4 in the intestine consists of two populations, one in the mucus which has an apparent generation time of 40 to 80 min and one in the luminal contents which is static.
通过与rRNA靶探针进行定量杂交以及落射荧光显微镜观察，估算了定殖于经链霉素处理小鼠大肠中的大肠杆菌BJ4的生长速率。测定了从盲肠黏液、盲肠内容物和粪便中分离出的细菌的核糖体含量，并将其与以确定速率在体外生长的细菌的核糖体含量进行关联。数据表明，大肠杆菌BJ4在肠道中总体生长速率较高。然而，考虑到盲肠黏液、盲肠内容物和粪便中的总肠道体积和细菌数量，我们认为肠道中的大肠杆菌BJ4由两个群体组成，一个群体存在于黏液中，其表观世代时间为40至80分钟，另一个群体存在于肠腔内容物中，处于静止状态。
