Poulsen L K, Lan F, Kristensen C S, Hobolth P, Molin S, Krogfelt K A
Department of Microbiology, Technical University of Denmark, Lyngby.
Infect Immun. 1994 Nov;62(11):5191-4. doi: 10.1128/iai.62.11.5191-5194.1994.
Fluorescent oligonucleotide probes targeting rRNA were used to develop an in situ hybridization technique by which the spatial distribution of Escherichia coli in the large intestines of streptomycin-treated mice was determined. Single E. coli cells were identified in thin frozen sections from the large intestines by the use of a probe specific for E. coli 23S rRNA. Furthermore, the total bacterial population was visualized with an rRNA probe targeting the domain Bacteria. By this technique, all E. coli cells were seen embedded in the mucosal material overlying the epithelial cells of the large intestine, and no direct attachment to the epithelium was observed.
靶向rRNA的荧光寡核苷酸探针被用于开发一种原位杂交技术，通过该技术确定链霉素处理小鼠大肠中大肠杆菌的空间分布。利用针对大肠杆菌23S rRNA的特异性探针，在大肠的薄冰冻切片中鉴定出单个大肠杆菌细胞。此外，用靶向细菌域的rRNA探针观察到总细菌群体。通过该技术，所有大肠杆菌细胞都嵌入在覆盖大肠上皮细胞的粘膜物质中，未观察到与上皮细胞的直接附着。
