一种来自甲基营养型放线菌甲醇拟无枝酸菌的能够进行位点特异性整合的质粒。
A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration.
作者信息
Vrijbloed J W, Madoń J, Dijkhuizen L
机构信息
Department of Microbiology, University of Groningen, Haren, The Netherlands.
出版信息
J Bacteriol. 1994 Nov;176(22):7087-90. doi: 10.1128/jb.176.22.7087-7090.1994.
Abstract
Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.
摘要
甲醇拟无枝酸菌含有一个13.3kb的质粒（pMEA300），它既可以以游离状态存在，也可以整合到一个独特的基因组位置。对一个2.1kb的pMEA300 DNA片段进行了测序，揭示了假定的附着点（attP）位点以及两个开放阅读框，xis和int，它们分别与编码切除酶和整合酶的基因具有相似性。
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