Gersell D J, Gingerich R L, Greider M H
Diabetes. 1979 Jan;28(1):11-5.
The regional concentrations of pancreatic polypeptide (PP), insulin, and glucagon and the cellular distribution of PP were studied in 13 human and nine canine pancreases by radioimmunoassay, immunoperoxidase localization, and cell quantitation. PP concentration was highest in both the uncinate process and the head of the human pancreas and in the right lobe of the canine pancreas. In contrast, glucagon and insulin levels were higher in the body and tail of both the human and canine pancreases. Human F-cells, which contain PP, were located primarily at the periphery of the islets, although a few F-cells were scattered throughout the ducts and acini. Canine F-cells were located in ducts, acini, and islets; the relative proportion of canine F-cells in the endocrine and exocrine tissues differed according to location. Cellular quantitation of F-cells in both species correlated significantly with the tissue concentration of PP in all regions studied, validating the use of morphometric techniques to quantitate the regional distribution of PP.
通过放射免疫测定、免疫过氧化物酶定位和细胞定量分析，对13例人胰腺和9例犬胰腺中胰多肽（PP）、胰岛素和胰高血糖素的区域浓度以及PP的细胞分布进行了研究。PP浓度在人胰腺的钩突和头部以及犬胰腺的右叶中最高。相比之下，人胰腺和犬胰腺的体部和尾部中胰高血糖素和胰岛素水平较高。含有PP的人F细胞主要位于胰岛的周边，尽管有一些F细胞散布在整个导管和腺泡中。犬F细胞位于导管、腺泡和胰岛中；犬F细胞在内分泌和外分泌组织中的相对比例因位置而异。在所有研究区域中，两个物种F细胞的细胞定量与PP的组织浓度均显著相关，这证实了使用形态计量技术定量PP区域分布的有效性。
