Marini P, Li S J, Gardiol D, Cronan J E, de Mendoza D
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
J Bacteriol. 1995 Dec;177(23):7003-6. doi: 10.1128/jb.177.23.7003-7006.1995.
The genes encoding two subunits of acetyl coenzyme A carboxylase, biotin carboxyl carrier protein, and biotin carboxylase have been cloned from Bacillus subtilis. DNA sequencing and RNA blot hybridization studies indicated that the B. subtilis accB homolog which encodes biotin carboxyl carrier protein, is part of an operon that includes accC, the gene encoding the biotin carboxylase subunit of acetyl coenzyme A carboxylase.
编码乙酰辅酶A羧化酶两个亚基、生物素羧基载体蛋白和生物素羧化酶的基因已从枯草芽孢杆菌中克隆出来。DNA测序和RNA印迹杂交研究表明,编码生物素羧基载体蛋白的枯草芽孢杆菌accB同源物是一个操纵子的一部分,该操纵子包括accC,即编码乙酰辅酶A羧化酶生物素羧化酶亚基的基因。