Lam K H, Schakenraad J M, Groen H, Esselbrugge H, Dijkstra P J, Feijen J, Nieuwenhuis P
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
J Biomed Mater Res. 1995 Aug;29(8):929-42. doi: 10.1002/jbm.820290804.
In this study, the influence of surface morphology and wettability of both degradable and nondegradable polymer films on the inflammatory response after subcutaneous implantation in the rat was investigated. Degradable non-porous, porous, and "combi" (porous with a nonporous layer on one side) poly(L-lactic acid) (PLLA) films and nondegradable polytetrafluoroethylene (PTFE) and (porous) expanded PTFE (e-PTFE) were used. Contact angles measurements indicate that PLLA is more hydrophillic than PTFE. Assessment of the inflammatory response was performed after various periods of implantation (up till 180 days), with both conventional light microscopy and immunohistochemistry using monoclonal antibodies (mAbs). The inflammatory response observed initially can largely be considered as part of the wound healing reaction, and up till day 40 the inflammatory response against PLLA was minimally more intense than against PTFE (porous as well as nonporous). From day 40 on, the PLLA films provoke a more intense inflammatory response as compared to the PTFE films. Both porous PLLA and the porous side of the "combi" PLLA film provoke a more intense inflammatory response than nonporous PLLA and the nonporous side of the "combi" PLLA film, respectively. In general, PTFE and e-PTFE films provoke an inflammatory response which is minimally more intense than the one provoked by the sham operation. Almost no ingrowth of tissue was observed in the porous e-PTFE films. In contrast, there was abundant tissue ingrowth in and an inflammatory response against porous PLLA. It can be concluded that biodegradable PLLA films provoke a more intense inflammatory response than nondegradable PTFE films. Also, porosity enhances the inflammatory response. However, porosity enhances the inflammatory response only when the wettability of a biomaterial permits cellular ingrowth.
在本研究中，研究了可降解和不可降解聚合物薄膜的表面形态和润湿性对大鼠皮下植入后炎症反应的影响。使用了可降解的无孔、多孔和“复合”（一侧有非多孔层的多孔）聚（L-乳酸）（PLLA）薄膜以及不可降解的聚四氟乙烯（PTFE）和（多孔）膨体聚四氟乙烯（e-PTFE）。接触角测量表明，PLLA比PTFE更亲水。在植入不同时间段（直至180天）后，使用单克隆抗体（mAb）通过传统光学显微镜和免疫组织化学对炎症反应进行评估。最初观察到的炎症反应在很大程度上可被视为伤口愈合反应的一部分，直到第40天，针对PLLA的炎症反应比针对PTFE（多孔和无孔）的炎症反应略微强烈一些。从第40天起，与PTFE薄膜相比，PLLA薄膜引发更强烈的炎症反应。多孔PLLA和“复合”PLLA薄膜的多孔侧分别比无孔PLLA和“复合”PLLA薄膜的无孔侧引发更强烈的炎症反应。一般来说，PTFE和e-PTFE薄膜引发的炎症反应比假手术引发的炎症反应略微强烈一些。在多孔e-PTFE薄膜中几乎未观察到组织长入。相反，多孔PLLA中有大量组织长入并引发炎症反应。可以得出结论，可生物降解的PLLA薄膜比不可降解的PTFE薄膜引发更强烈的炎症反应。此外，孔隙率会增强炎症反应。然而，只有当生物材料的润湿性允许细胞长入时，孔隙率才会增强炎症反应。
