Söderlund M, Brown C S, Spaan W J, Hedman L, Hedman K
Department of Virology, University of Helsinki, Finland.
J Infect Dis. 1995 Dec;172(6):1431-6. doi: 10.1093/infdis/172.6.1431.
Temporal reactivities of IgG towards native and linear antigenic determinants in assembled capsids or isolated structural proteins of human parvovirus B19 were measured by an epitope type-specific IgG EIA and by immunoblots. Antigens used were baculovirus-expressed B19 capsids composed of the proteins VP1 and VP2 in their native proportion, VP2 alone, or a prokaryotic VP1 fusion protein. Follow-up sera after primary infection were compared with samples from previously infected persons. The IgG responses during acute and early convalescence phases were directed towards both conformational and linear epitopes of VP2. The antibodies against the linear VP2 epitopes disappeared abruptly within 6 months; however, the conformational VP2 antibodies persisted. The epitope type-specific IgG reactivity of VP1 was strikingly different from that of VP2. On the basis of these results, two novel tests were developed for patient diagnosis. Both tests are suitable for verifying the time of human parvovirus infection.
通过表位类型特异性IgG酶免疫测定法和免疫印迹法，检测了IgG对人细小病毒B19组装衣壳或分离结构蛋白中天然和线性抗原决定簇的时间反应性。所用抗原为杆状病毒表达的B19衣壳，其由天然比例的蛋白VP1和VP2、单独的VP2或原核VP1融合蛋白组成。将初次感染后的随访血清与先前感染者的样本进行比较。急性和早期恢复期的IgG反应针对VP2的构象和线性表位。针对线性VP2表位的抗体在6个月内突然消失；然而，构象VP2抗体持续存在。VP1的表位类型特异性IgG反应性与VP2明显不同。基于这些结果，开发了两种用于患者诊断的新检测方法。这两种检测方法都适用于验证人细小病毒感染的时间。
