Munakata H, Kayada Y, Kawahara M, Sakamoto T, Yoshiga K, Takada K
Department of Oral and Maxillofacial Surgery I, Hiroshima University School of Dentistry, Japan.
Int J Oral Maxillofac Surg. 1995 Jun;24(3):216-20. doi: 10.1016/s0901-5027(06)80131-1.
An auxiliary method for determination of chemosensitivity with the subrenal capsule assay (SRCA) was developed in which the specific activity of succinate dehydrogenase (SD) of tumor implanted beneath the renal capsule is measured. The appropriate conditions for measuring the specific activity of SD were determined. The chemosensitivity of tumors, derived from six xenograft lines originating from oral squamous cell carcinomas, to peplomycin (PEP), cisplatin (CDDP), and 5-fluorouracil (5-FU) were evaluated by the SRCA and the nude mouse assay (NMA). The chemosensitivity evaluated by NMA displayed a higher degree of correlation with that determined by the improved SRCA than with that determined by the conventional SRCA. The correlations between overall accuracy of prediction with the NMA and those with the conventional SRCA and the improved SRCA were 72.2% and 88.9%, respectively. These findings suggest that our new assay may be useful for evaluation of chemosensitivity in the SRCA.
开发了一种辅助方法，通过肾包膜下测定法（SRCA）来确定化学敏感性，该方法是测量植入肾包膜下的肿瘤中琥珀酸脱氢酶（SD）的比活性。确定了测量SD比活性的合适条件。通过SRCA和裸鼠测定法（NMA）评估了源自6种口腔鳞状细胞癌异种移植系的肿瘤对培洛霉素（PEP）、顺铂（CDDP）和5-氟尿嘧啶（5-FU）的化学敏感性。与传统SRCA相比，NMA评估的化学敏感性与改进后的SRCA测定的化学敏感性具有更高的相关性。NMA预测的总体准确性与传统SRCA和改进后的SRCA预测的总体准确性之间的相关性分别为72.2%和88.9%。这些发现表明，我们的新测定法可能有助于评估SRCA中的化学敏感性。
