肾包膜下移植法作为原发性食管鳞状细胞癌的化学敏感性试验

Subrenal capsule assay as a chemosensitivity test for primary esophageal squamous cell carcinoma.

作者信息

Sunagawa M, Suzuki T, Ito K, Endo M

机构信息

First Department of Surgery, Tokyo Medical and Dental University, School of Medicine, Japan.

出版信息

J Surg Oncol. 1991 Feb;46(2):71-6. doi: 10.1002/jso.2930460202.

DOI:10.1002/jso.2930460202

PMID:1704079

Abstract

The efficiency of the subrenal capsule assay (SRCA) was studied with fresh tissue of esophageal squamous cell carcinoma. The day-to-day changes in 10 carcinoma cases were evaluated for 9 days. The cancer cells continued to proliferate from the 3rd to the 7th day after the implantation and then decreased. The host reaction was recognized histologically from the 3rd or 4th day to the 9th day. However, the immune reaction did not significantly influence the evaluation of SRCA until the 7th day. The immunohistochemical staining with anti-bromodeoxyuridine monoclonal antibody revealed the existence of cancer cells at the DNA synthesizing stage (S stage) in the graft until the 7th day. In chemosensitivity test by SRCA, 21 patients were studied, and all were evaluable. 5-FU administration produced a response in 8/21 cases (38.1%), VDS in 8/21 (38.1%), and CDDP in 3/21 (14.3%). Used in combination, CDDP + VDS was effective in 7/18 cases (38.9%) and CDDP + BLM in 6/18 cases (33.3%).

摘要

采用食管鳞状细胞癌新鲜组织研究了肾包膜下接种法（SRCA）的效率。对10例癌组织的每日变化进行了9天的评估。癌细胞在植入后第3天至第7天持续增殖，然后减少。宿主反应从第3天或第4天到第9天在组织学上可识别。然而，直到第7天免疫反应才对SRCA的评估产生显著影响。用抗溴脱氧尿苷单克隆抗体进行免疫组织化学染色显示，直到第7天移植物中仍存在处于DNA合成期（S期）的癌细胞。在通过SRCA进行的化疗敏感性试验中，研究了21例患者，所有患者均可评估。5-氟尿嘧啶给药在8/21例（38.1%）中产生反应，长春地辛在8/21例（38.1%）中产生反应，顺铂在3/21例（14.3%）中产生反应。联合使用时，顺铂+长春地辛在7/18例（38.9%）中有效，顺铂+博来霉素在6/18例（33.3%）中有效。