Schütze H, Enzmann P J, Kuchling R, Mundt E, Niemann H, Mettenleiter T C
Federal Research Centre for Virus Diseases of Animals, Institute of Molecular and Cellular Virology, Friedrich-Loeffler-Institutes, Insel-Riems, Germany.
J Gen Virol. 1995 Oct;76 ( Pt 10):2519-27. doi: 10.1099/0022-1317-76-10-2519.
The complete nucleotide sequence of the genome of the fish rhabdovirus infectious haematopoietic necrosis virus (IHNV) has been determined after cDNA cloning of the viral genomic RNA. Sequence analysis showed the presence of six open reading frames encoding the nucleoprotein N, the matrix proteins M1 and M2, the glycoprotein G, a so-called non-structural protein NV, and the RNA polymerase L. The genome organization is 3'N-M1-M2-G-NV-L 5'. The extreme 5' and 3' ends of the genome were sequenced after RNA ligation or RACE. Prokaryotic expression products of the open reading frames predicted to encode the matrix proteins M1 and M2, the glycoprotein G and the NV protein reacted with rabbit anti-IHNV serum thereby confirming their identity. This is the first complete nucleotide sequence of a fish rhabdovirus. Knowledge of the complete sequence is an essential prerequisite for future manipulation of the genome and also serves to provide gene- and protein specific reagents for use in further examination of the replication of the fish rhabdoviruses.
在对鱼类弹状病毒传染性造血器官坏死病毒（IHNV）的基因组RNA进行cDNA克隆后，已确定了其基因组的完整核苷酸序列。序列分析显示存在六个开放阅读框，分别编码核蛋白N、基质蛋白M1和M2、糖蛋白G、一种所谓的非结构蛋白NV以及RNA聚合酶L。基因组结构为3'N-M1-M2-G-NV-L 5'。通过RNA连接或RACE对基因组的极端5'和3'末端进行了测序。预测编码基质蛋白M1和M2、糖蛋白G以及NV蛋白的开放阅读框的原核表达产物与兔抗IHNV血清发生反应，从而证实了它们的身份。这是鱼类弹状病毒的首个完整核苷酸序列。完整序列的知识是未来对基因组进行操作的必要前提，也有助于提供基因和蛋白质特异性试剂，用于进一步研究鱼类弹状病毒的复制。
