Schütze H, Mundt E, Mettenleiter T C
Institute of Molecular and Cellular Virology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany.
Virus Genes. 1999;19(1):59-65. doi: 10.1023/a:1008140707132.
The complete nucleotide sequence of the fish rhabdovirus viral hemorrhagic septicemia virus (VHSV) has been determined. The genome comprises 11158 bases and contains six long open reading frames encoding the nucleoprotein N, phosphoprotein P, matrix protein M, glycoprotein G, nonstructural viral protein NV, and polymerase L. Genes are arranged in the order 3'-N-P-M-G-NV-L-5'. The exact 3' and 5' ends were determined after RNA-oligonucleotide ligation or RACE. They show inverse complementarity as in other rhabdovirus genomes. Nucleotide and deduced amino acid sequences exhibit significant homology to corresponding sequences in the related fish rhabdovirus infectious hematopoietic necrosis virus.
鱼类弹状病毒——病毒性出血性败血症病毒（VHSV）的完整核苷酸序列已被确定。基因组由11158个碱基组成，包含六个长开放阅读框，分别编码核蛋白N、磷蛋白P、基质蛋白M、糖蛋白G、非结构病毒蛋白NV和聚合酶L。基因排列顺序为3'-N-P-M-G-NV-L-5'。通过RNA寡核苷酸连接或RACE确定了精确的3'和5'末端。它们与其他弹状病毒基因组一样呈现反向互补性。核苷酸和推导的氨基酸序列与相关鱼类弹状病毒——传染性造血坏死病毒的相应序列具有显著同源性。
