Hiraguchi E
Second Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Kaku Igaku. 1995 May;32(5):453-63.
Blood clearance and hepatic uptake parameters of 99mTc-DTPA-galactosyl human serum albumin (99mTc-GSA) were evaluated in two groups of rats, normal liver group and CCl4 induced chronic liver injury group. In each group, four subgroups were classified as follows: simple laparotomy (LAP), hepatic artery ligation (HAL), portal vein ligation (PVL) and both hepatic artery and portal vein ligation (HAL + PVL). 99mTc-GSA was intravenously injected (50 micrograms/100 g B.W.) to rats. Heart and liver were targetted as region of interest and time activity curves (TACs) were obtained. The blood clearance parameters (HH4 and Kd) and the hepatic uptake parameters (LHL4 and Ku) were calculated from these TACs. In normal liver group, the mean HH4 of LAP was significantly different from those of PVL and HAL + PVL. Similarly, the other three parameters (LHL4, Kd and Ku) of LAP were significantly different from those of PVL and HAL + PVL. CCl4 induced chronic liver injury group showed significant difference on all four parameters in four subgroups. Hepatic tissue blood flow rates (HTBFR) calculated by Laser Doppler flowmeter correlated well with 99mTc-GSA parameters in both groups. These results suggest that 99mTc-GSA is useful to estimate hepatic blood flow not only in rats with normal liver, but also in rats with CCl4 induced chronic liver injury.
在正常肝脏组和四氯化碳诱导的慢性肝损伤组两组大鼠中评估了99mTc-DTPA-半乳糖基人血清白蛋白（99mTc-GSA）的血液清除和肝脏摄取参数。在每组中，四个亚组分类如下：单纯剖腹术（LAP）、肝动脉结扎（HAL）、门静脉结扎（PVL）以及肝动脉和门静脉双结扎（HAL + PVL）。将99mTc-GSA静脉注射给大鼠（50微克/100克体重）。将心脏和肝脏作为感兴趣区域，并获得时间-活度曲线（TACs）。从这些TACs计算出血液清除参数（HH4和Kd）以及肝脏摄取参数（LHL4和Ku）。在正常肝脏组中，LAP的平均HH4与PVL和HAL + PVL的平均HH4有显著差异。同样，LAP的其他三个参数（LHL4、Kd和Ku）与PVL和HAL + PVL的参数有显著差异。四氯化碳诱导的慢性肝损伤组在四个亚组的所有四个参数上均显示出显著差异。通过激光多普勒血流仪计算的肝组织血流率（HTBFR）与两组中的99mTc-GSA参数相关性良好。这些结果表明，99mTc-GSA不仅对正常肝脏的大鼠，而且对四氯化碳诱导的慢性肝损伤大鼠估计肝血流都很有用。
