Zweygarth E, Kaminsky R, Moloo S K
Kenya Trypanosomiasis Research Institute, KETRI, Kikuyu.
Onderstepoort J Vet Res. 1994 Jun;61(2):189-91.
Two Trypanosoma vivax stocks were initiated in culture with tsetse or culture-derived metacyclics. They were propagated axenically as bloodstream trypomastigotes at 35 degrees C in 4% CO2 in air. Populations of trypanosomes were incubated with various concentrations of antitrypanosomal compounds, namely diminazene aceturate, quinapyramine sulphate, DL-alpha-difluoromethylornithine, isometamidium chloride, suramin and melCy. Growth was monitored after 24 h of incubation and the growth inhibition was calculated. All six drugs tested showed little effect upon the growth of the parasite populations. These results indicate that a 24-h growth-inhibition test was not suitable for determining the drug susceptibility of T. vivax stocks in vitro. Neither did the results correlate with those obtained with susceptible or resistant stocks of T.b. brucei, T.b. evansi or T. simiae described in the literature, or with the results of these two T. vivax stocks tested in cattle.
用采采蝇或培养来源的循环后期锥虫在培养基中启动了两株间日锥虫原种。它们在35摄氏度、空气中4%二氧化碳的条件下，以无共生体的血流型锥鞭毛体形式进行繁殖。将锥虫群体与不同浓度的抗锥虫化合物（即乙酰氨基阿苯达唑、硫酸喹嘧胺、DL-α-二氟甲基鸟氨酸、氯胺苯脒、苏拉明和melCy）一起孵育。孵育24小时后监测生长情况并计算生长抑制率。所测试的所有六种药物对寄生虫群体的生长几乎没有影响。这些结果表明，24小时生长抑制试验不适用于在体外确定间日锥虫原种的药物敏感性。这些结果也与文献中描述的布氏锥虫、伊氏锥虫或西氏锥虫敏感或抗性原种所获得的结果不相关，也与在牛身上测试的这两株间日锥虫原种的结果不相关。
