Zweygarth E, Moloo S K, Kaminsky R
Kenya Trypanosomiasis Research Institute, KETRI, Kikuyu.
Acta Trop. 1993 Sep;54(3-4):301-8. doi: 10.1016/0001-706x(93)90102-h.
Two Trypanosoma simiae stocks were initiated in culture with tsetse-derived metacyclics. They were propagated axenically as trypomastigote forms at 35 degrees C in 4% CO2 in air. Populations of trypanosomes were incubated with various concentrations of antitrypanosomal compounds. Growth was monitored after 24 h of incubation and the growth inhibition was calculated. Diminazene aceturate, quinapyramine sulphate, DL-alpha-difluoromethylornithine, and Ro 15-0216 showed activity against the stocks. Suramin and Mel Cy showed little effect upon the growth of the parasite populations. Isometamidium chloride gave questionable results in the 24 h growth inhibition test, but the results of a long-term viability assay indicated some degree of drug resistance (or drug tolerance). The results obtained herein correlate with observations obtained from in vivo studies in pigs. It is thus concluded that in many cases the cryptic nature of T. simiae rather than drug resistance is responsible for the failure of chemotherapy of simiae-trypanosomiasis in pigs.
用采采蝇来源的循环后期锥虫在培养物中启动了两株猴锥虫原种。它们在35℃、4%二氧化碳的空气中以锥鞭毛体形式进行无共生繁殖。将不同浓度的抗锥虫化合物与锥虫群体一起孵育。孵育24小时后监测生长情况并计算生长抑制率。乙酰氨基阿维菌素、硫酸喹嘧胺、DL-α-二氟甲基鸟氨酸和Ro 15-0216对这些原种显示出活性。苏拉明和Mel Cy对寄生虫群体的生长几乎没有影响。氯化异美汀在24小时生长抑制试验中给出的结果存疑，但长期活力测定的结果表明存在一定程度的耐药性（或药物耐受性）。本文获得的结果与在猪体内研究中观察到的结果相关。因此得出结论，在许多情况下，猴锥虫的隐匿性而非耐药性是猪的猴锥虫病化疗失败的原因。
