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西方许旺酵母LEU2同源基因的克隆与测序

Cloning and sequencing of the LEU2 homologue gene of Schwanniomyces occidentalis.

作者信息

Iserentant D, Verachtert H

机构信息

Laboratory of Industrial Microbiology and Biochemistry, Catholic University of Leuven, Louvain, Belgium.

出版信息

Yeast. 1995 Apr 30;11(5):467-73. doi: 10.1002/yea.320110510.

DOI:10.1002/yea.320110510
PMID:7597851
Abstract

A gene that complements the leu2 mutation of Saccharomyces cerevisiae has been cloned from Schwanniomyces occidentalis. The gene codes for a protein of 379 amino acids. As expected for a Schwanniomyces gene, it has a high AT content, which is also reflected in the codon usage. The sequence homology with other known leu2 complementing genes is low.

摘要

一个与酿酒酵母亮氨酸2(leu2)突变互补的基因已从西方许旺酵母中克隆出来。该基因编码一种由379个氨基酸组成的蛋白质。正如对一个许旺酵母基因所预期的那样,它具有较高的AT含量,这在密码子使用中也有所体现。与其他已知的亮氨酸2互补基因的序列同源性较低。

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