Kikuchi Y, Kitazawa Y, Shimatake H, Yamamoto M
Department of Molecular Biology, Toho University, School of Medicine, Tokyo, Japan.
Curr Genet. 1988 Oct;14(4):375-9. doi: 10.1007/BF00419995.
A DNA fragment which carries the leu1 gene encoding beta-isopropylmalate dehydrogenase in Schizosaccharomyces pombe has been isolated by complementation of an E. coli leuB mutation. This 1.5 kb DNA fragment complements not only the S. pombe leu1 mutation, but also the S. cerevisiae leu2 mutation. The nucleotide sequence of the essential part of the leu1 gene and its flanking regions was determined. This sequence contains an open reading frame of 371 codons, from which a protein having a Mr = 39,732 can be predicted. The deduced amino acid sequence and its codon usage were compared with those of the S. cerevisiae LEU2 protein. The cloned DNA will be a useful marker when transforming S. pombe.
通过互补大肠杆菌leuB突变，已分离出携带编码粟酒裂殖酵母β-异丙基苹果酸脱氢酶的leu1基因的DNA片段。这个1.5 kb的DNA片段不仅能互补粟酒裂殖酵母leu1突变，还能互补酿酒酵母leu2突变。测定了leu1基因及其侧翼区域关键部分的核苷酸序列。该序列包含一个371个密码子的开放阅读框，由此可预测出一个Mr = 39,732的蛋白质。将推导的氨基酸序列及其密码子使用情况与酿酒酵母LEU2蛋白进行了比较。克隆的DNA在转化粟酒裂殖酵母时将是一个有用的标记。
