Sosis M B, Braverman B, Villaflor E
Department of Anesthesiology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA.
Anesth Analg. 1995 Jul;81(1):132-4. doi: 10.1097/00000539-199507000-00026.
We determined whether propofol, thiopental, or 0.9% saline would serve as a growth medium for Candida albicans. In Part I, we investigated whether opening 20 propofol ampules would cause glass particles from the exterior of the ampule to fall into the emulsion and contaminate it. Each ampule was painted with red fingernail polish and its contents were passed through filter paper after it was opened in a routine manner. In Part II, a sample from a colony of C albicans was added to sterile vials containing 20 mL of either 0.9% saline, 1% propofol, or 2.5% thiopental. A 1-microL sample from each vial was then plated onto Sabaroud's dextrose plus brain-heart infusion (SABHI) agar at the following times after inoculation: 0, 3, 6, 16, and 24 h. The plates were incubated at 35 degrees C for 24 h and the number of colony-forming units counted. The filtration of two of the painted ampules in Part I revealed red glass fragments. In Part II, the saline and thiopental solutions did not increase the number of colonies of C. albicans by 24 h. However, the propofol, after a latent period between 6 and 16 h, supported the growth of C. albicans at a rapid rate. Our investigation shows that glass particles from the exterior of a propofol ampule can contaminate its contents when the ampule is opened in a routine manner. Furthermore, propofol provides an excellent growth medium for C. albicans. Thiopental and saline showed no growth. We conclude that the ability of propofol to grow C. albicans necessitates rigorous standards of sterility in its handling.
我们确定了丙泊酚、硫喷妥钠或0.9%生理盐水是否会成为白色念珠菌的生长培养基。在第一部分中，我们研究了打开20支丙泊酚安瓿瓶是否会导致安瓿瓶外部的玻璃颗粒落入乳剂并对其造成污染。每个安瓿瓶都涂上红色指甲油，按常规方式打开后，其内容物通过滤纸过滤。在第二部分中，将一份来自白色念珠菌菌落的样本添加到含有20 mL 0.9%生理盐水、1%丙泊酚或2.5%硫喷妥钠的无菌小瓶中。然后在接种后的以下时间，从每个小瓶中取1 μL样本接种到沙氏葡萄糖加脑心浸液（SABHI）琼脂平板上：0、3、6、16和24小时。平板在35摄氏度下孵育24小时，并计算菌落形成单位的数量。第一部分中两个涂漆安瓿瓶的过滤显示有红色玻璃碎片。在第二部分中，到24小时时，生理盐水和硫喷妥钠溶液并未增加白色念珠菌的菌落数量。然而，丙泊酚在6至16小时的潜伏期后，能快速支持白色念珠菌的生长。我们的研究表明，按常规方式打开丙泊酚安瓿瓶时，安瓿瓶外部的玻璃颗粒会污染其内容物。此外，丙泊酚为白色念珠菌提供了极佳的生长培养基。硫喷妥钠和生理盐水未显示有生长情况。我们得出结论，丙泊酚支持白色念珠菌生长的特性要求在其处理过程中要有严格的无菌标准。
