Magnuson K, Hellström S, Magnuson B
Department of Otorhinolaryngology, University of Umeå, Sweden.
Eur Arch Otorhinolaryngol. 1995;252(2):76-82. doi: 10.1007/BF00168024.
Healthy adult laboratory rats were exposed to alternating negative pressure and atmospheric pressure to replicate the clinical situation found in patients with chronic sniffing habits and chronic middle ear disease. The rats were placed in a box in which the pressure changed at intervals of 30 s between atmospheric pressure and a negative pressure of -3 kPa. This was repeated continuously for periods of 3 and 7 days. At completion of the experimental period, all rats had a normal otomicroscopic status. However, histological studies demonstrated that the pars flaccida was wrinkled and the loose connective tissue contained large fibroblasts with their long axes lying in a disorganized manner. The cells of the keratinizing epithelium were thicker than normal and mitoses were seen. Epithelial crypts filled with keratin were numerous along the epithelium. In the pars tensa, all layers were thicker than normal. These findings demonstrate that repeated pressure loading can create structural changes in the tympanic membrane.
将健康成年实验大鼠暴露于交替的负压和大气压下，以模拟慢性吸鼻习惯和慢性中耳疾病患者的临床情况。将大鼠置于一个盒子中，压力每隔30秒在大气压和-3 kPa的负压之间变化。这种情况持续重复3天和7天。在实验期结束时，所有大鼠的耳显微镜检查状态正常。然而，组织学研究表明，松弛部出现皱纹，疏松结缔组织中有大型成纤维细胞，其长轴排列紊乱。角化上皮细胞比正常情况更厚，可见有丝分裂。沿上皮有许多充满角蛋白的上皮隐窝。在紧张部，所有层均比正常情况厚。这些发现表明，反复的压力负荷可导致鼓膜出现结构变化。
