通过对5个核苷酸位置进行聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析对ABO血型进行基因分型。
Genotyping of ABO blood groups by PCR and RFLP analysis of 5 nucleotide positions.
作者信息
Fukumori Y, Ohnoki S, Shibata H, Yamaguchi H, Nishimukai H
机构信息
Research Department, Osaka Red Cross Blood Center, Japan.
出版信息
Int J Legal Med. 1995;107(4):179-82. doi: 10.1007/BF01428401.
The genotyping of ABO blood groups was performed using the polymerase chain reaction (PCR) method. The 4 DNA fragments containing the nucleotide position 261, 526, 703 and 796 of cDNA from A-transferase were amplified by PCR, and the amplified DNA subjected to restriction fragment length polymorphism (RFLP) analysis. The different nucleotide at position 803 was clearly distinguished by electrophoresis of the PCR products amplified with allele-specific primers. By analyzing the electrophoresis patterns, ABO genotyping was conclusively accomplished. The frequencies of ABO genotypes found in Japanese blood donors with A and B phenotypes were as follows: in the phenotype A group, AA = 19.8% and AO = 80.2%; and in the phenotype B group, BB = 12.8% and BO = 87.2%.
采用聚合酶链反应（PCR）方法进行ABO血型基因分型。通过PCR扩增包含A转移酶cDNA核苷酸位置261、526、703和796的4个DNA片段，并对扩增的DNA进行限制性片段长度多态性（RFLP）分析。用等位基因特异性引物扩增的PCR产物经电泳可清晰区分803位的不同核苷酸。通过分析电泳图谱，最终完成ABO基因分型。在日本具有A和B血型表型的献血者中发现的ABO基因型频率如下：在A血型表型组中，AA = 19.8%，AO = 80.2%；在B血型表型组中，BB = 12.8%，BO = 87.2%。
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