Nishimukai H, Fukumori Y, Okiura T, Yuasa I, Shinomiya T, Ohnoki S, Shibata H, Vogt U
Department of Legal Medicine, School of Medicine, Ehime University, Japan.
Int J Legal Med. 1996;109(2):90-3. doi: 10.1007/BF01355523.
Genotypes of the ABO blood group system were studied by PCR-RFLP analysis of the eight polymorphic nucleotide positions (nps) 261, 467, 526, 646, 703, 796, 802 and 803 of the cDNA from A transferase. In 169 unrelated German individuals, 17 genotypes were found and the calculated allele frequencies of A(Pro), A(Leu), B, O(T), O(A) and O2 were 0.2130, 0.0770, 0.0473, 0.4260, 0.2160 and 0.0207, respectively. These frequency data may provide useful additional information for disputed paternity and stain testing. A variant O allele, O2, was fout at a polymorphic frequency. As the nucleotide (np 261) of the O2 allele is the same as that of A and B alleles, the analysis of at least three nucleotide positions, i.e. nps 261, 526 and 802, is necessary to avoid mistyping of the ABO genotype.
通过对A转移酶cDNA的8个多态性核苷酸位点（nps）261、467、526、646、703、796、802和803进行PCR-RFLP分析，研究了ABO血型系统的基因型。在169名无亲缘关系的德国个体中，发现了17种基因型，计算出的A(Pro)、A(Leu)、B、O(T)、O(A)和O2等位基因频率分别为0.2130、0.0770、0.0473、0.4260、0.2160和0.0207。这些频率数据可能为有争议的亲子鉴定和污渍检测提供有用的额外信息。发现了一个变异的O等位基因O2，其频率具有多态性。由于O2等位基因的核苷酸（np 261）与A和B等位基因的相同，因此至少分析三个核苷酸位点，即nps 261、526和802，对于避免ABO基因型的误判是必要的。
