Bruno L, Kirberg J, von Boehmer H
Basel Institute for Immunology, Switzerland.
Immunity. 1995 Jan;2(1):37-43. doi: 10.1016/1074-7613(95)90077-2.
We have studied memory in T cell receptor (TCR) transgenic mice expressing a Db-restricted TCR specific for the male peptide (H-Y). CD8+ T cells from female TCR transgenic C57BL/6 (B6) mice were activated by transferring them into X-irradiated male (B6 x bm12)F1 hybrid recipients. Subsequently, they were highly purified by cell sorting and transferred for various lengths of time into female B6 nu/nu recipient mice. Other nu/nu recipient mice received highly purified naive T cells expressing the transgenic TCR. The functional potential of naive and "memory" T cells was analyzed by stimulation with male cells in vivo. The results show that memory cells can be derived from activated T cells and persist in the absence of antigen for at least 13 weeks. Naive and memory T cells differ in that memory T cells give a more vigorous and sustained response than naive T cells.
我们研究了表达针对雄性肽（H-Y）的Db限制性T细胞受体（TCR）的T细胞受体转基因小鼠的记忆。将来自雌性TCR转基因C57BL/6（B6）小鼠的CD8+ T细胞转移到经X射线照射的雄性（B6×bm12）F1杂交受体小鼠中，使其活化。随后，通过细胞分选对它们进行高度纯化，并将其转移到雌性B6 nu/nu受体小鼠中不同时长。其他nu/nu受体小鼠接受表达转基因TCR的高度纯化的初始T细胞。通过在体内用雄性细胞刺激来分析初始T细胞和“记忆”T细胞的功能潜力。结果表明，记忆细胞可源自活化的T细胞，并且在没有抗原的情况下持续存在至少13周。初始T细胞和记忆T细胞的不同之处在于，记忆T细胞比初始T细胞产生更强烈和持续的反应。
