Schulte-Merker S, van Eeden F J, Halpern M E, Kimmel C B, Nüsslein-Volhard C
Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Tübingen, Germany.
Development. 1994 Apr;120(4):1009-15. doi: 10.1242/dev.120.4.1009.
The mouse T (Brachyury) gene is required for normal mesoderm development and the extension of the body axis. Recently, two mutant alleles of a zebrafish gene, no tail (ntl), have been isolated (Halpern, M. E., Ho., R. K., Walker, C. and Kimmel, C. B. (1993) Cell 75, 99-111). ntl mutant embryos resemble mouse T/T mutant embryos in that they lack a differentiated notochord and the caudal region of their bodies. We report here that this phenotype is caused by mutation of the zebrafish homologue of the T gene. While ntl embryos express mutant mRNA, they show no nuclear protein product. Later, expression of mRNA in mutants, but not in wild types, is greatly reduced along the dorsal midline where the notochord normally forms. This suggests that the protein is required for maintaining transcription of its own gene.
小鼠T（短尾）基因对于正常中胚层发育和体轴延伸是必需的。最近，斑马鱼无尾（ntl）基因的两个突变等位基因已被分离出来（哈尔彭，M.E.，何，R.K.，沃克，C.和金梅尔，C.B.（1993年）《细胞》75卷，99 - 111页）。ntl突变胚胎与小鼠T/T突变胚胎相似，因为它们缺乏分化的脊索以及身体的尾部区域。我们在此报告，这种表型是由T基因的斑马鱼同源物的突变引起的。虽然ntl胚胎表达突变型mRNA，但它们没有核蛋白产物。后来，突变体中mRNA的表达在正常形成脊索的背中线沿线大幅减少，而野生型中则没有。这表明该蛋白质对于维持其自身基因的转录是必需的。
