Jasoni C L, Walker M B, Morris M D, Reh T A
Department of Biological Structure, University of Washington, Seattle 98195, USA.
Development. 1994 Apr;120(4):769-83. doi: 10.1242/dev.120.4.769.
We have identified a basic helix-loop-helix encoding cDNA from embryonic chicken retina which shares sequence similarity with the achaete-scute family of genes of Drosophila. The deduced amino acid sequence of this chicken achaete-scute homolog (CASH-1) is identical, over the region encoding the basic helix-loop-helix domain, to the recently identified mammalian achaete-scute homolog (MASH-1) and to the Xenopus homolog (XASH1), and 70% identical, over the same region, to Drosophila achaete-scute complex members. The expression of CASH-1 is restricted to subsets of neuronal progenitor cells in the developing chicken nervous system, similar in distribution to that reported for MASH-1 and XASH1. In addition, in situ localization in the retina reveals a dynamic character of expression of the gene in a particular region of the CNS, and suggests that the expression of CASH-1 may be important in defining a particular stage in the progenitor cell necessary for the differentiation of particular neuronal phenotypes.
我们从鸡胚视网膜中鉴定出一种编码基本螺旋-环-螺旋结构的cDNA，它与果蝇的achaete-scute基因家族具有序列相似性。这种鸡achaete-scute同源物（CASH-1）推导的氨基酸序列，在编码基本螺旋-环-螺旋结构域的区域，与最近鉴定出的哺乳动物achaete-scute同源物（MASH-1）和非洲爪蟾同源物（XASH1）相同，在同一区域与果蝇achaete-scute复合体成员有70%的同源性。CASH-1的表达局限于发育中的鸡神经系统的神经元祖细胞亚群，其分布与报道的MASH-1和XASH1相似。此外，视网膜中的原位定位揭示了该基因在中枢神经系统特定区域表达的动态特征，并表明CASH-1的表达可能在定义特定神经元表型分化所需的祖细胞的特定阶段中起重要作用。
