Pasricha P J, Tietjen T G, Kalloo A N
Section of Therapeutic Endoscopy, Johns Hopkins Hospital, Baltimore, MD, USA.
Endoscopy. 1995 Jan;27(1):70-2. doi: 10.1055/s-2007-1005636.
Biliary manometry, the "gold standard" for the diagnosis of sphincter of Oddi dysfunction, is associated with technical and methodological problems. The lack of a suitable experimental model has hindered efforts to solve these problems. We report here on the first practical animal model for endoscopic biliary manometry, similar in technique to the procedure in humans. Piglets were sedated and intubated with a standard human duodenoscope. A standard water-perfused manometry catheter was inserted into the bile duct. The biliary sphincter was identified by a zone of high-pressure activity with superimposed phasic contractions. The sphincter responded normally to the administration of cholecystokinin and morphine by relaxation and contraction, respectively. This model should be useful for training in biliary manometry, and facilitate technical innovations in the field. Since it is relatively atraumatic, it may also be better than existing surgical models for studying the normal physiology and pharmacology of the sphincter of Oddi.
胆管测压作为诊断Oddi括约肌功能障碍的“金标准”，存在技术和方法上的问题。缺乏合适的实验模型阻碍了解决这些问题的努力。我们在此报告首个用于内镜下胆管测压的实用动物模型，其技术与人类操作相似。给仔猪注射镇静剂后，用标准的人类十二指肠镜进行插管。将标准的水灌注测压导管插入胆管。通过高压活动区域叠加相位收缩来识别胆管括约肌。括约肌分别对注射胆囊收缩素和吗啡正常地作出松弛和收缩反应。该模型应有助于胆管测压的培训，并促进该领域的技术创新。由于其创伤相对较小，在研究Oddi括约肌的正常生理学和药理学方面，它可能也优于现有的手术模型。
