利用限制性内切酶标记基因组扫描揭示SMXA重组近交系小鼠品系的遗传图谱。

Genetic profile of the SMXA recombinant inbred mouse strains revealed with restriction landmark genomic scanning.

作者信息

Mori M, Akiyoshi S, Mizuno Y, Okuizumi H, Okazaki Y, Hayashizaki Y, Nishimura M

机构信息

Genome Science Laboratory, RIKEN Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba City, Ibaraki 305-0074, Japan.

出版信息

Mamm Genome. 1998 Sep;9(9):695-709. doi: 10.1007/s003359900850.

DOI:10.1007/s003359900850

PMID:9716654

Abstract

We have applied the restriction landmark genomic scanning (RLGS) method to the SMXA recombinant inbred (RI) mouse strain set to reveal its detailed genetic profile. A total of 663 polymorphic RLGS spot loci were identified, 576 of which were assigned to chromosomes. Strain distribution patterns (SDPs) at 55 microsatellite marker loci were also obtained. As a result, the total number of loci with distinct SDPs on chromosomes increased to 400. These loci were dispersed on all chromosomes, except for the Chromosome (Chr) Y, and effectively covered the genome with an average spacing of 4 cM. The SMXA RI strain set, hereby, would be of value for genetic study.

摘要

我们已将限制性内切酶切位点基因组扫描（RLGS）方法应用于SMXA重组近交（RI）小鼠品系组，以揭示其详细的遗传图谱。共鉴定出663个多态性RLGS斑点位点，其中576个被定位到染色体上。还获得了55个微卫星标记位点的菌株分布模式（SDP）。结果，染色体上具有不同SDP的位点总数增加到400个。这些位点分布在除Y染色体外的所有染色体上，平均间距为4 cM，有效地覆盖了基因组。因此，SMXA RI品系组对遗传学研究具有重要价值。