Greger V, Knoll J H, Woolf E, Glatt K, Tyndale R F, DeLorey T M, Olsen R W, Tobin A J, Sikela J M, Nakatsu Y
Genetics Division, Children's Hospital, Boston, MA 02115, USA.
Genomics. 1995 Mar 20;26(2):258-64. doi: 10.1016/0888-7543(95)80209-5.
GABAA receptors are heterooligomeric ligand-gated ion channels that mediate the effect of the inhibitory neurotransmitter gamma-aminobutyric acid. The GABAA receptors consist of at least 15 different receptor subunits that can be classified into 5 subfamilies (alpha, beta, gamma, delta, rho) on the basis of sequence similarity. Chromosomal mapping studies have revealed that several of the GABAA receptor subunit genes appear to be organized as clusters. One such cluster, which consists of the GABAA receptor beta 3 (GABRB3) and alpha 5 (GABRA5) subunit genes, is located in chromosome 15q11-q13. It is shown here that the GABAA receptor gamma 3 subunit gene (GABRG3) also maps to this region. Lambda and P1 phage clones surrounding both ends of GABRG3 were isolated; the clones derived from the 5' end of GABRG3 were linked to an existing phage contig spanning the 3' end of GABRA5. The two genes are located within 35 kb of each other and are transcribed in the same orientation.
GABAA受体是异源寡聚体配体门控离子通道，介导抑制性神经递质γ-氨基丁酸的作用。GABAA受体由至少15种不同的受体亚基组成，根据序列相似性可分为5个亚家族（α、β、γ、δ、ρ）。染色体定位研究表明，几个GABAA受体亚基基因似乎是以簇的形式组织的。其中一个这样的簇由GABAA受体β3（GABRB3）和α5（GABRA5）亚基基因组成，位于15号染色体q11-q13区域。本文显示GABAA受体γ3亚基基因（GABRG3）也定位于该区域。分离出围绕GABRG3两端的λ和P1噬菌体克隆；来自GABRG3 5'端的克隆与跨越GABRA5 3'端的现有噬菌体重叠群相连。这两个基因位于彼此35 kb范围内，且转录方向相同。
