大肠杆菌K-12的narX、narL、narP和narQ基因的表达：调节因子的调控
Expression of the narX, narL, narP, and narQ genes of Escherichia coli K-12: regulation of the regulators.
作者信息
Darwin A J, Stewart V
机构信息
Section of Microbiology, Cornell University, Ithaca, New York 14853-8101, USA.
出版信息
J Bacteriol. 1995 Jul;177(13):3865-9. doi: 10.1128/jb.177.13.3865-3869.1995.
Abstract
The products of four Escherichia coli genes (narX, narL, narQ, and narP) regulate anaerobic respiratory gene expression in response to nitrate and nitrite. We used lacZ gene and operon fusions to monitor the expression of these nar regulatory genes in response to different growth conditions. Maximal expression of the narXL operon required molybdate, nitrate, and integration host factor. Expression of the narP and narQ genes was weakly repressed by nitrate. The NarL and NarP proteins were required for full nitrate induction of narXL operon expression, whereas the nitrate repression of narP and narQ expression was mediated solely by the NarL protein. narXL operon expression was unaffected by anaerobiosis, whereas expression of narP and narQ was induced approximately fourfold. The Fnr and ArcA proteins were not required for this anaerobic induction.
摘要
四种大肠杆菌基因（narX、narL、narQ和narP）的产物可响应硝酸盐和亚硝酸盐调节厌氧呼吸基因的表达。我们使用lacZ基因和操纵子融合来监测这些nar调控基因在不同生长条件下的表达。narXL操纵子的最大表达需要钼酸盐、硝酸盐和整合宿主因子。narP和narQ基因的表达受到硝酸盐的微弱抑制。NarL和NarP蛋白是narXL操纵子表达完全被硝酸盐诱导所必需的，而narP和narQ表达的硝酸盐抑制仅由NarL蛋白介导。narXL操纵子的表达不受厌氧状态的影响，而narP和narQ的表达则被诱导约四倍。Fnr和ArcA蛋白不是这种厌氧诱导所必需的。
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