Ueguchi C, Shiozawa T, Kakeda M, Yamada H, Mizuno T
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
J Bacteriol. 1995 Jul;177(13):3894-6. doi: 10.1128/jb.177.13.3894-3896.1995.
The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations. In particular, the cbpA dnaJ double-null mutant was found to exhibit severe defects in cell growth, namely, a very narrow temperature range for growth, a defect in cell division, and susceptibility to killing by carbon starvation. These phenotypes are very similar to those reported for dnaK null mutants but not to those of dnaJ null mutants. Our results are best interpreted by assuming that CbpA is capable of compensating for DnaJ for cell growth and thus that the function(s) of CbpA is closely related to that of DnaJ.
CbpA蛋白是大肠杆菌DnaJ分子伴侣的类似物。为深入了解CbpA的功能，我们特别参照dnaK和dnaJ无效突变的情况，研究了cbpA无效突变的性质。特别是，发现cbpA dnaJ双无效突变体在细胞生长方面表现出严重缺陷，即生长的温度范围非常狭窄、细胞分裂存在缺陷以及对碳饥饿杀伤敏感。这些表型与报道的dnaK无效突变体的表型非常相似，但与dnaJ无效突变体的表型不同。我们的结果最好通过假设CbpA能够补偿DnaJ对细胞生长的作用来解释，因此CbpA的功能与DnaJ的功能密切相关。
