Shimakoshi Y, Sano K, Nakano T, Nakamura T, Ohshiba S, Katsu K, Nakai M
Second Department of Internal Medicine, Osaka Medical College, Japan.
Kansenshogaku Zasshi. 1995 May;69(5):532-8. doi: 10.11150/kansenshogakuzasshi1970.69.532.
We devised a micro-suspension-test to evaluate disinfectants against human immunodeficiency virus type-1 (HIV-1) and confirmed its reliability. Suspensions of persistently HIV-1-infected Molt-4 cells were used as targets of disinfectants and residual infectivity was measured by an infectivity assay: after cocultivation with uninfected Molt-4 cells reverse transcriptase activity (RTA) in the supernatant and giant cell formation (GCF) were monitored. Our new infectivity assay consists of a short-term assay, that is RTA and GCF monitoring on the second day of co-culture, and a long-term assay, that is RTA monitoring up to the 28th day of co-culture. The sensitivity of the short-term assay was 1 x 10(3) infected cells and that of the long-term assay 1 x 10(1) infected cells. All the chemical disinfectants examined in this study showed dose- and time-dependent inactivation of HIV-1. By 5-minute contact with ethanol, glutaraldehyde, formalin, sodium hypochlorite and povidone-iodine, HIV-1 was effectively inactivated at concentrations of 20, 0.01, 5, 0.05 and 0.1%, respectively. Since the micro-suspension-test is easy and sensitive, we recommend it as a method for evaluating disinfectants against HIV-1.
我们设计了一种微悬浮试验来评估消毒剂对1型人类免疫缺陷病毒（HIV-1）的效果，并证实了其可靠性。持续感染HIV-1的Molt-4细胞悬液用作消毒剂的作用靶标，通过感染性测定来测量残留感染性：在与未感染的Molt-4细胞共培养后，监测上清液中的逆转录酶活性（RTA）和巨细胞形成（GCF）。我们新的感染性测定包括一个短期测定，即在共培养第二天监测RTA和GCF，以及一个长期测定，即在共培养第28天监测RTA。短期测定的灵敏度为1×10³个感染细胞，长期测定的灵敏度为1×10¹个感染细胞。本研究中检测的所有化学消毒剂对HIV-1均显示出剂量和时间依赖性的灭活作用。与乙醇、戊二醛、福尔马林、次氯酸钠和聚维酮碘接触5分钟后，HIV-1在浓度分别为20%、0.01%、5%、0.05%和0.1%时被有效灭活。由于微悬浮试验简便且灵敏，我们推荐将其作为评估针对HIV-1消毒剂的一种方法。
