Kinoshita Y, Monafo W W
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Microvasc Res. 1995 Mar;49(2):190-200. doi: 10.1006/mvre.1995.1015.
The purpose of this study was to explore further adrenergic regulation of peripheral nerve blood flow (NBF) before and after surgical stress. We applied norepinephrine (NE) to one sciatic nerve of rats either immediately after the nerve had been exposed or 2 hr later. Regional NBF was then measured using [14C]butanol distribution. Biceps femoris muscle flow (MBF) was measured simultaneously. The effect of NE on endoneurial NBF was also measured. Contralateral limbs were controls. Control NBF was 11.9 +/- 1.9 total, 9.1 +/- 1.5 endoneurial (units: ml min-1 100 g-1). If 10(-5) M NE was applied immediately postexposure, neither total nor endoneurial NBF was affected. If 10(-5) M NE was applied 2 hr postexposure, however, total NBF on the control side was 21 +/- 2.3 vs 8.3 +/- 1.2 on the NE side (P < 0.001). In all surgically exposed limbs, MBF was elevated and fell sharply in response to 10(-5) M NE. We conclude that under basal conditions, neither total nor endoneurial NBF is affected by topical NE. When NBF is already elevated from surgical trauma, however, NE reduces it.
本研究的目的是进一步探讨手术应激前后外周神经血流（NBF）的肾上腺素能调节。我们在大鼠坐骨神经暴露后立即或2小时后将去甲肾上腺素（NE）应用于一侧坐骨神经。然后使用[14C]丁醇分布测量局部NBF。同时测量股二头肌血流（MBF）。还测量了NE对神经内膜NBF的影响。对侧肢体作为对照。对照NBF总量为11.9±1.9，神经内膜为9.1±1.5（单位：ml·min-1·100g-1）。如果在暴露后立即应用10^(-5)M NE，总NBF和神经内膜NBF均不受影响。然而，如果在暴露后2小时应用10^(-5)M NE，对照侧的总NBF为21±2.3，而NE侧为8.3±1.2（P<0.001）。在所有手术暴露的肢体中，MBF升高，并对10^(-5)M NE迅速下降。我们得出结论，在基础条件下，局部NE既不影响总NBF也不影响神经内膜NBF。然而，当NBF因手术创伤已经升高时，NE会使其降低。
