Kinoshita Y, Monafo W W
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.
Exp Neurol. 1994 Feb;125(2):296-301. doi: 10.1006/exnr.1994.1032.
The factors affecting peripheral nerve blood flow (NBF) are not well defined. We used [14C]butanol distribution to quantitate NBF in rat sciatic nerve following either (1) simple surgical exposure (EXP) or (2) EXP plus nerve mobilization and isolation (ISOL). Undisturbed contralateral nerves were controls. The epineurial contribution to NBF after EXP was also assessed. Results (units: ml.min-1 x 100 g-1): Within 1 to 6 h after EXP (N = 7), NBF was 22.1 +/- 2.7 vs 11.9 +/- 1.5 control (P < 0.01). Seven to 24 h after EXP (N = 6), NBF was unchanged from control. Two hours following EXP, both endoneurial NBF and total (epineurial plus endoneurial) NBF were elevated (P < 0.05, P < 0.02, respectively). Within 48 h after ISOL (N = 6) NBF was 30.3 +/- 3.9 vs 13.2 +/- 2.1 control (P < 0.005). Three to seven days after ISOL (N = 8), NBF was unchanged from control.
Surgical trauma induces a brisk flow response in peripheral nerve that is proportionate in duration to the trauma severity. Measurements of NBF after EXP or ISOL are therefore potentially misleading if the changes in NBF due to the trauma are not accounted for.
影响周围神经血流量（NBF）的因素尚未明确。我们使用[14C]丁醇分布法来定量大鼠坐骨神经的NBF，方法如下：（1）单纯手术暴露（EXP）；（2）EXP加神经游离和分离（ISOL）。未受干扰的对侧神经作为对照。同时评估了EXP后神经外膜对NBF的贡献。结果（单位：ml·min-1×100 g-1）：EXP后1至6小时（N = 7），NBF为22.1±2.7，而对照为l1.9±1.5（P<0.01）。EXP后7至24小时（N = 6），NBF与对照无变化。EXP后2小时，神经内膜NBF和总（神经外膜加神经内膜）NBF均升高（分别为P<0.05，P<0.02）。ISOL后48小时内（N = 6），NBF为30.3±3.9，而对照为13.2±2.1（P<0.005）。ISOL后3至7天（N = 8），NBF与对照无变化。
手术创伤在外周神经中诱发快速血流反应，其持续时间与创伤严重程度成正比。因此，如果不考虑创伤引起的NBF变化，EXP或ISOL后NBF的测量可能会产生误导。
