针对欧洲鳗鲡（Anguilla anguilla L., 1758）免疫球蛋白的单克隆抗体的制备、特性鉴定及应用
Production, characterisation and applicability of monoclonal antibodies to European eel (Anguilla anguilla L., 1758) immunoglobulin.
作者信息
van der Heijden M H, Rooijakkers J B, Booms G H, Rombout J H, Boon J H
机构信息
Department of Fish Culture and Fisheries, Wageningen Agricultural University, Netherlands.
出版信息
Vet Immunol Immunopathol. 1995 Mar;45(1-2):151-64. doi: 10.1016/0165-2427(94)05335-p.
Monoclonal antibodies (mAbs) to European eel (Anguilla anguilla L., 1758) immunoglobulin (Ig) were produced, characterised and tested for applicability in a number of immuno(cyto)chemical assays. The selected mAbs, WEI 1 and WEI 2, were specifically reactive with Ig heavy and light chain, respectively. WEI 1 appeared to react with all or nearly all Ig molecules, B cells and plasma cells. WEI 2 was reactive with a subpopulation of those cells, indicating that European eel possesses at least two antigenically different light chain types. Both mAbs could be used for detection of antigen-specific antibodies in plasma by means of an enzyme-linked immunosorbent assay (ELISA).
制备了针对欧洲鳗鲡（Anguilla anguilla L.，1758）免疫球蛋白（Ig）的单克隆抗体（mAb），对其进行了表征，并测试了它们在多种免疫（细胞）化学分析中的适用性。所选的单克隆抗体WEI 1和WEI 2分别与Ig重链和轻链发生特异性反应。WEI 1似乎与所有或几乎所有的Ig分子、B细胞和浆细胞发生反应。WEI 2与这些细胞的一个亚群发生反应，这表明欧洲鳗鲡至少拥有两种抗原性不同的轻链类型。两种单克隆抗体均可通过酶联免疫吸附测定（ELISA）用于检测血浆中的抗原特异性抗体。
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