针对狒狒IgG重链和轻链表位的小鼠单克隆抗体的制备与特性分析

Production and characterization of murine monoclonal antibodies specific for baboon IgG heavy and light chain epitopes.

作者信息

Shearer M H, Jenson H B, Carey K D, Chanh T C, Kennedy R C

机构信息

Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.

出版信息

J Med Primatol. 1994 Sep;23(7):382-7. doi: 10.1111/j.1600-0684.1994.tb00124.x.

DOI:10.1111/j.1600-0684.1994.tb00124.x

PMID:7537335

Abstract

We have generated a panel of murine monoclonal antibodies (MAbs) that recognize baboon IgG epitopes. The reactivity of the MAbs with IgG from other primate species was also examined. Specificity for IgG heavy (H) or light (L) chain epitopes was determined by Western blot analysis. The H chain-specific MAbs were analyzed for IgG subclass specificity and the L chain-specific MAbs for reactivity with baboon IgM and polymeric sIgA. Finally, an ELISA was developed to demonstrate the utility of the MAbs in analysis of humoral immune responses in baboons.

摘要

我们已经制备了一组可识别狒狒IgG表位的小鼠单克隆抗体（MAb）。还检测了这些单克隆抗体与其他灵长类物种IgG的反应性。通过蛋白质印迹分析确定了对IgG重链（H）或轻链（L）表位的特异性。分析了重链特异性单克隆抗体的IgG亚类特异性，以及轻链特异性单克隆抗体与狒狒IgM和聚合sIgA的反应性。最后，开发了一种酶联免疫吸附测定法（ELISA），以证明这些单克隆抗体在分析狒狒体液免疫反应中的实用性。