Motomura N, Eishi K, Kitoh H, Kawazoe K, Kitoh Y, Kawashima Y, Oka T
Department of Cardiovascular Surgery, National Cardiovascular Center, Osaka, Japan.
Cardiovasc Surg. 1995 Apr;3(2):231-4. doi: 10.1016/0967-2109(95)90902-h.
A key point in the establishment of a tissue cryopreservation system is to maintain cell viability for an extended period. The viability of the tissue cells in the authors' cryopreservation system was examined, and the first clinical application reported. The tissue culture method demonstrated 95% of rat aortas (21 of 22 specimens) and 88% of human cardiac valves (seven of eight) to be viable after 6.4 months and 5.8 months of cryopreservation, respectively. The first clinical case, a 54-year-old man with aortic valve replacement and complications of aortitis, underwent successful aortic root replacement. Whereas the use of allografts in aortitis cases is controversial, such an approach was assumed reasonable because the mechanical stress between the native aortic annulus and the inserted valve could be reduced by aortic root replacement with the allograft.
建立组织冷冻保存系统的一个关键点是长时间维持细胞活力。对作者冷冻保存系统中的组织细胞活力进行了检测，并报告了首例临床应用情况。组织培养方法显示，分别在冷冻保存6.4个月和5.8个月后，22个大鼠主动脉标本中有21个（95%）、8个人心脏瓣膜标本中有7个（88%）的组织细胞仍具有活力。首例临床病例是一名54岁男性，患有主动脉瓣置换术和主动脉炎并发症，成功接受了主动脉根部置换术。虽然在主动脉炎病例中使用同种异体移植物存在争议，但这种方法被认为是合理的，因为用同种异体移植物进行主动脉根部置换可以减轻天然主动脉瓣环与植入瓣膜之间的机械应力。
