Hutchinson A M
Department of Natural Products, Glaxo Research and Development Ltd., Greenford, Middlesex, UK.
Mol Biotechnol. 1995 Feb;3(1):47-54. doi: 10.1007/BF02821334.
Optical biosensors, based on evanescent wave technology, are analytical devices that measure the interactions between biomolecules in real time, without the need for any labels. Specific ligands are immobilized to a sensor surface, and a solution of receptor or antibody is injected over the top. Binding is measured by recording changes in the refractive index, caused by the molecules interacting near the sensor surface within the evanescent field. Evanescent wave-based biosensors are being used to study an increasing number of applications in the life sciences, including the binding and dissociation kinetics of antibodies and receptor-ligand pairs, protein-DNA and DNA-DNA interactions, epitope mapping, phage display libraries, and whole cell- and virus-protein interactions. There are currently four commercially available evanescent wave biosensors on the market. This article describes the technology behind their sensing techniques, as well as the range of applications in which they are employed.
基于倏逝波技术的光学生物传感器是一种分析设备，可实时测量生物分子之间的相互作用，无需任何标记。将特定配体固定在传感器表面，然后将受体或抗体溶液注入其上方。通过记录折射率的变化来测量结合情况，这种变化是由倏逝场内传感器表面附近相互作用的分子引起的。基于倏逝波的生物传感器正被用于生命科学领域越来越多的应用研究，包括抗体与受体 - 配体对的结合和解离动力学、蛋白质 - DNA和DNA - DNA相互作用、表位作图、噬菌体展示文库以及全细胞与病毒 - 蛋白质相互作用。目前市场上有四种可商购的倏逝波生物传感器。本文介绍了其传感技术背后的原理，以及它们的应用范围。
