Campbell G, Göring H, Lin T, Spana E, Andersson S, Doe C Q, Tomlinson A
Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Development. 1994 Oct;120(10):2957-66. doi: 10.1242/dev.120.10.2957.
We report the identification of RK2, a glial-specific homeodomain protein. RK2 is localized to the nucleus of virtually all embryonic and imaginal glial cells, with the exception of midline glia. Embryos mutant for the gene encoding RK2 are embryonic lethal but normal for early gliogenesis (birth, initial divisions and migration of glia) and axonogenesis (neuronal pathfinding and fasciculation). However, later in development, there are significantly fewer longitudinal glia that are spatially disorganized; in addition, there is a slight disorganization of axon fascicles and a defective nerve cord condensation. This suggests that RK2 is not required for early glial determination, but rather for aspects of glial differentiation or function that are required for embryonic viability.
我们报告了胶质细胞特异性同源结构域蛋白RK2的鉴定。RK2定位于几乎所有胚胎期和成虫期胶质细胞的细胞核中，但中线胶质细胞除外。编码RK2的基因突变的胚胎在胚胎期致死，但早期胶质细胞生成（胶质细胞的产生、初始分裂和迁移）和轴突生成（神经元路径寻找和束状化）正常。然而，在发育后期，纵向胶质细胞数量显著减少且空间分布紊乱；此外，轴突束略有紊乱，神经索凝聚存在缺陷。这表明RK2对于早期胶质细胞的确定并非必需，而是对于胚胎存活所必需的胶质细胞分化或功能的某些方面是必需的。
