Horgan G W, Glasbey C A
Scottish Agricultural, Statistics Service, Edinburgh.
Electrophoresis. 1995 Mar;16(3):298-305. doi: 10.1002/elps.1150160149.
The information in electrophoretic gels can be extracted visually, or by using specialised computer hardware and software which treat the gels as digital images. We discuss issues which arise in applying digital image analysis to electrophoresis, and comment on the range of computer packages available. We also illustrate how image analysis can make gel interpretation easier and more reliable. Methods covered include gel registration and warping, mathematical morphology and deconvolution to sharpen images.
电泳凝胶中的信息可以通过肉眼提取,也可以使用专门的计算机硬件和软件来提取,这些硬件和软件将凝胶视为数字图像。我们讨论了将数字图像分析应用于电泳时出现的问题,并对可用的计算机软件包范围进行了评论。我们还举例说明了图像分析如何使凝胶解读更轻松、更可靠。涵盖的方法包括凝胶配准和变形、数学形态学以及去卷积以锐化图像。