Rakin A, Heesemann J
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.
FEMS Microbiol Lett. 1995 Jun 15;129(2-3):287-92. doi: 10.1111/j.1574-6968.1995.tb07594.x.
The fyuA/irp2 gene cluster, which is part of the Yersinia pestis pigmentation (pgm) locus encoding genes involved in iron uptake and virulence, is present in all pesticin-sensitive bacteria. In Y. enterocolitica biotype 1B strains (serotypes O8, O20, O21), the fyuA/irp2 gene cluster carries an insertion of a novel repetitive sequence, IS1328. It was also found in the genome of Y. enterocolitica O5 (biotype 1A) and O13 (biotype 1B), but not in pesticin-sensitive Y. pseudotuberculosis O1 and Escherichia coli Phi. The 1353-bp repetitive element has 12-bp perfect inverted terminal repeats. A single open reading frame is capable of encoding a 334-amino acid polypeptide. IS1328 DNA has high homology with the DNA sequences located downstream of the aggR gene from the enteroaggregative E. coli (EAggEC), to the region of the R751 plasmid flanking Tn501, to the sequence following the merR gene of S. marcescens pDU1358 plasmid, and to the sequences of K. pneumoniae plasmid pCFF04. The putative polypeptide has 36.4% identity with the transposase encoded by the Coxiella burnetii IS1111a insertion sequence. The IS1328 insertion sequence could be responsible for the deletions of the fyuA/irp2 gene cluster observed in Y. enterocolitica O8 and could represent a member of a new group of widely distributed repetitive elements.
fyuA/irp2基因簇是鼠疫耶尔森菌色素沉着（pgm）位点的一部分，该位点编码参与铁摄取和毒力的基因，存在于所有对杀鼠疫菌素敏感的细菌中。在小肠结肠炎耶尔森菌生物型1B菌株（血清型O8、O20、O21）中，fyuA/irp2基因簇携带一个新的重复序列ISl328的插入。在小肠结肠炎耶尔森菌O5（生物型1A）和O13（生物型1B）的基因组中也发现了它，但在对杀鼠疫菌素敏感的假结核耶尔森菌O1和大肠杆菌Phi中未发现。这个1353bp的重复元件有12bp的完美反向末端重复序列。一个单一的开放阅读框能够编码一个334个氨基酸的多肽。IS1328 DNA与来自肠聚集性大肠杆菌（EAggEC）的aggR基因下游的DNA序列、与侧翼Tn501的R751质粒区域、与粘质沙雷氏菌pDU1358质粒的merR基因后的序列以及与肺炎克雷伯菌质粒pCFF04的序列具有高度同源性。推测的多肽与由伯氏考克斯氏体IS1111a插入序列编码的转座酶有36.4%的同一性。ISl328插入序列可能是小肠结肠炎耶尔森菌O8中观察到的fyuA/irp2基因簇缺失的原因，并且可能代表一组广泛分布的新的重复元件中的一个成员。
