Carniel E, Guilvout I, Prentice M
Unité de Bactériologie Moléculaire et Médicale, Laboratoire des Yersinia, Institut Pasteur, Paris, France.
J Bacteriol. 1996 Dec;178(23):6743-51. doi: 10.1128/jb.178.23.6743-6751.1996.
Pathogenic Yersinia spp. can be subdivided into highly pathogenic (high-pathogenicity) and low-pathogenicity strains. Several genes specific for the high-pathogenicity strains are clustered on a chromosomal fragment designated a "high-pathogenicity island" (HPI). In the present work, the HPI of biotype 1B strain Ye 8081 of Y. enterocolitica was characterized. We demonstrate important differences from the HPI of Y. pestis. The HPI of Y. enterocolitica is smaller (45 kb) and is not flanked by insertion sequences. A copy of the gene coding for the tRNA-Asn is present at one extremity of the HPI and may, as in uropathogenic Escherichia coli, participate in the excision of the island. In addition to the genes encoding the yersiniabactin-pesticin receptor and the high-molecular-weight protein 2, four repeated sequences are present on the HPI of Y. enterocolitica. At least two of them are insertion elements: previously described IS1328 and newly characterized IS1400. Comparison of the HPI of strain Ye 8081 with that of other Y. enterocolitica strains of biotype 1B indicates that most of the island is conserved, apart from 15 kb at the left-hand end which is variable, especially in the region where three repeated sequences are clustered.
致病性耶尔森氏菌属可细分为高致病性（高致病力）和低致病力菌株。几种高致病力菌株特有的基因聚集在一个被称为“高致病力岛”（HPI）的染色体片段上。在本研究中，对小肠结肠炎耶尔森氏菌1B生物型菌株Ye 8081的HPI进行了特征分析。我们证明了它与鼠疫耶尔森氏菌的HPI存在重要差异。小肠结肠炎耶尔森氏菌的HPI较小（45 kb），且两侧没有插入序列。编码tRNA-Asn的基因拷贝存在于HPI的一端，可能像在尿路致病性大肠杆菌中一样，参与该岛的切除。除了编码yersiniabactin-鼠疫菌素受体和高分子量蛋白2的基因外，小肠结肠炎耶尔森氏菌的HPI上还存在四个重复序列。其中至少两个是插入元件：先前描述的IS1328和新鉴定的IS1400。将菌株Ye 8081的HPI与其他1B生物型小肠结肠炎耶尔森氏菌菌株的HPI进行比较表明，除了左端15 kb可变区域外，该岛的大部分区域是保守的，特别是在三个重复序列聚集的区域。
