Calvin Koons M D, Blumenthal R M
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008, USA.
Gene. 1995 May 19;157(1-2):73-9. doi: 10.1016/0378-1119(94)00618-3.
Plasmid pPvu1 from Proteus vulgaris carries the genes of the PvuII restriction-modification system [Blumenthal et al., J. Bacteriol. 164 (1985) 501-509]. This report focuses on physical and functional features of the 4.84-kb plasmid, which shows a composite genetic architecture. Plasmid pPvu1 has a replication origin and an incompatibility locus that each function in Escherichia coli, and an apparent cer recombination site. The replication origin includes a possible RNA I gene, and the incompatibility locus closely resembles a rom gene. These loci show substantial sequence similarity to corresponding loci from the E. coli plasmids P15A, ColEI and pSC101, and closely flank the PvuII genes. The close association between a recombinational locus and the PvuII genes has implications for their mobility.
来自普通变形杆菌的质粒pPvu1携带PvuII限制修饰系统的基因[布卢门撒尔等人，《细菌学杂志》164(1985)501 - 509]。本报告聚焦于这个4.84 kb质粒的物理和功能特征，该质粒呈现出复合遗传结构。质粒pPvu1有一个复制起点和一个不相容位点，它们在大肠杆菌中均有功能，还有一个明显的cer重组位点。复制起点包含一个可能的RNA I基因，不相容位点与一个rom基因非常相似。这些位点与大肠杆菌质粒P15A、ColEI和pSC101的相应位点有显著的序列相似性，并且紧密位于PvuII基因两侧。一个重组位点与PvuII基因的紧密关联对它们的移动性有影响。
