Hsd质粒pECO29的完整核苷酸序列及其功能区域的鉴定
Complete nucleotide sequence of the Hsd plasmid pECO29 and identification of its functional regions.
作者信息
Zakharova M V, Pertzev A V, Kravetz A N, Beletskaya I V, Shlyapnikov M G, Solonin A S
机构信息
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russian Federation.
出版信息
Biochim Biophys Acta. 1998 Jun 16;1398(2):106-12. doi: 10.1016/s0167-4781(98)00051-7.
The complete nucleotide sequence of the Hsd plasmid pECO29 has been determined. The plasmid DNA consists of 3895 base pairs. These include 4 genes and 5 sites. Two genes encoding the proteins (restriction endonuclease and DNA methyltransferase) have been fully characterized. The pECO29 comprises a Co1El-type replication system coding for untranslated genes RNAI and RNAII, the emr recombination site containing palindromic sequences and involved in stable maintenance of the plasmid, two pseudo oriT sites homologous to the oriT site of R64 and F plasmids, as well as the bom locus of a Co1El-like plasmid. There are no genes involved in the mobilization of pECO29 plasmid.
已确定Hsd质粒pECO29的完整核苷酸序列。该质粒DNA由3895个碱基对组成。其中包括4个基因和5个位点。两个编码蛋白质（限制性内切核酸酶和DNA甲基转移酶）的基因已得到充分表征。pECO29包含一个编码非翻译基因RNAI和RNAII的ColE1型复制系统、含有回文序列且参与质粒稳定维持的emr重组位点、两个与R64和F质粒的oriT位点同源的假oriT位点，以及一个ColE1样质粒的bom位点。不存在参与pECO29质粒转移的基因。
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