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PvuII核酸内切酶基因pvuIIR及其调控基因pvuIIC的序列与特性分析

Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene.

作者信息

Tao T, Blumenthal R M

机构信息

Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.

出版信息

J Bacteriol. 1992 May;174(10):3395-8. doi: 10.1128/jb.174.10.3395-3398.1992.

DOI:10.1128/jb.174.10.3395-3398.1992
PMID:1577705
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206011/
Abstract

An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.

摘要

一个开放阅读框与pvuIIR部分重叠，遗传学证据表明这个名为pvuIIC的开放阅读框指定了pvuIIR的一个正调控因子（T. 陶、J. C. 伯恩和R. M. 布卢门撒尔，《细菌学杂志》173:1367 - 1375，1991年）。pvuIIC的可诱导构建体产生了预期大小的蛋白质。PvuII C的作用位点似乎位于pvuIIC自身内部；因此，pvuIIC可能是一个独立的调控盒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c795/206011/e5d18060b0db/jbacter00076-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c795/206011/e5d18060b0db/jbacter00076-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c795/206011/e5d18060b0db/jbacter00076-0311-a.jpg

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