大鼠JAK2（一种蛋白酪氨酸激酶）编码基因的克隆。

Cloning of the gene encoding rat JAK2, a protein tyrosine kinase.

作者信息

Duhé R J, Rui H, Greenwood J D, Garvey K, Farrar W L

机构信息

Biological Carcinogenesis and Development Program, Program Resources, Inc./DynCorp, Frederick, MD 21702-1201, USA.

出版信息

Gene. 1995 Jun 9;158(2):281-5. doi: 10.1016/0378-1119(95)00041-4.

DOI:10.1016/0378-1119(95)00041-4

PMID:7607555

Abstract

A complete cDNA clone encoding the rat JAK2 protein tyrosine kinase was isolated from an Nb2-SP (rat pre-T lymphoma cell line) cDNA library. The nucleotide (nt) and deduced amino acid (aa) sequences for this clone were determined and an open reading frame of 3399 bp, encoding a protein of a deduced mass of 130 kDa, was found. The coding regions of the rat and murine Jak2 clones share 93.4% nt identity and 97.1% aa identity. Northern analysis demonstrated that the 5-kb mRNA is highly abundant in brain and spleen, less abundant in skeletal muscle and testis, and detectable in kidney, heart, lung and liver. Translation of the rat Jak2 mRNA in rabbit reticulocytes results in a protein which is specifically immunoprecipitated by antibodies (Ab) recognizing JAK2, but not by Ab recognizing JAK1.

摘要

从Nb2-SP（大鼠前T淋巴瘤细胞系）cDNA文库中分离出一个编码大鼠JAK2蛋白酪氨酸激酶的完整cDNA克隆。测定了该克隆的核苷酸（nt）和推导的氨基酸（aa）序列，发现一个3399 bp的开放阅读框，编码一个推导质量为130 kDa的蛋白质。大鼠和小鼠Jak2克隆的编码区核苷酸同一性为93.4%，氨基酸同一性为97.1%。Northern分析表明，5-kb的mRNA在脑和脾中高度丰富，在骨骼肌和睾丸中较少，在肾、心、肺和肝中可检测到。大鼠Jak2 mRNA在兔网织红细胞中的翻译产生一种蛋白质，该蛋白质被识别JAK2的抗体（Ab）特异性免疫沉淀，但不被识别JAK1的Ab免疫沉淀。