Kon H, Matsushita M, Takahashi M, Nishikawa M, Taguchi K, Koike M, Kamachi H, Uchino J
First Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Int J Artif Organs. 1995 Jan;18(1):27-33.
To assess the possibility of using hepatocytes from ischemic liver, as a bioreactor of a hybrid artificial liver, we investigated the influence of warm ischemia on the isolation and culture of hepatocytes in rats. Warm ischemia was induced by clamping the liver hilus and the animals were divided into 3 groups according to the duration of ischemia: group A (no ischemia), group B (10 minutes) and group C (20 minutes). Hepatocytes were isolated by the collagenase perfusion method and cultured for 5 days. The yield and viability of the isolated hepatocytes were lower in group C. Rate of attachment was decreased as the duration of ischemia increased. There was no significant difference observed in functions in culture. Sufficient hepatocytes, as a bioreactor, can be isolated and cultured from warm ischemic liver within 10 minutes. Though the number of available hepatocytes were diminished, hepatocytes procured from longer warm ischemic liver could be utilized as a bioreactor.
为了评估使用缺血肝脏中的肝细胞作为混合人工肝生物反应器的可能性，我们研究了热缺血对大鼠肝细胞分离和培养的影响。通过夹闭肝门诱导热缺血，并根据缺血持续时间将动物分为3组：A组（无缺血）、B组（10分钟）和C组（20分钟）。采用胶原酶灌注法分离肝细胞并培养5天。C组分离出的肝细胞产量和活力较低。随着缺血持续时间的增加，贴壁率降低。培养中的功能未观察到显著差异。在10分钟内可从热缺血肝脏中分离并培养出足够数量的肝细胞作为生物反应器。虽然可用肝细胞数量减少，但从较长热缺血肝脏中获取的肝细胞仍可作为生物反应器使用。
