Nishikawa M, Uchino J, Matsushita M, Takahashi M, Taguchi K, Koike M, Kamachi H, Kon H
First Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Artif Organs. 1996 Feb;20(2):169-77. doi: 10.1111/j.1525-1594.1996.tb00723.x.
With a view toward furthering the development of artificial liver systems, we have been culturing hepatocytes in vitro. The object of this research was to investigate the ideal conditions of oxygen tension for the efficient functioning of hepatocytes. Viable hepatocytes isolated from rat livers were cultured under five different oxygen tensions: 5, 10, 20, 50 and 90% O2. DNA contents, gluconeogenesis, urea synthesis, adenosine triphosphate (ATP) levels, and lipid peroxidation of hepatocytes were evaluated. Under the 5% oxygen conditions, the function of hepatocytes was very inferior and was accompanied by a low ATP level. However, hepatocytes cultured under 90% oxygen tension functioned less effectively than the control (20% O2) with elevated lipid peroxidation. The data in this study suggest that the optimum oxygen condition for cultured hepatocytes is 10 approximately 50%, and that especially under conditions of 20% oxygen tension, i.e., that of the ordinary atmosphere, hepatocytes can function most effectively.
为了进一步推动人工肝系统的发展，我们一直在体外培养肝细胞。本研究的目的是探讨氧气张力的理想条件，以实现肝细胞的高效功能。从大鼠肝脏分离出的活肝细胞在五种不同的氧气张力下进行培养：5%、10%、20%、50%和90% O₂。评估了肝细胞的DNA含量、糖异生、尿素合成、三磷酸腺苷（ATP）水平和脂质过氧化情况。在5%氧气条件下，肝细胞功能非常差，且ATP水平较低。然而，在90%氧气张力下培养的肝细胞功能不如对照组（20% O₂）有效，脂质过氧化水平升高。本研究数据表明，培养肝细胞的最佳氧气条件约为10%至50%，特别是在20%氧气张力（即普通大气条件）下，肝细胞能最有效地发挥功能。
