Hamoen L W, Eshuis H, Jongbloed J, Venema G, van Sinderen D
Department of Genetics, University of Groningen, Haren, The Netherlands.
Mol Microbiol. 1995 Jan;15(1):55-63. doi: 10.1111/j.1365-2958.1995.tb02220.x.
The valine-activation domain-encoding portion of the srfA locus (srfA-d4) is not only involved in the non-ribosomal synthesis of surfactin, but is also required for the regulation of competence development. In this study we show that impairment of the adenylation activity of the valine-activating domain did not affect competence development. Deletion analysis and complementation studies delineated the competence-required portion of srfA-d4 to a 168 bp fragment, which contains a small open reading frame (ORF), designated comS, encoding a polypeptide of 46 amino acids, embedded within, but translated in, a frame different from that of srfA-d4. Introduction of an amber mutation in the comS-coding frame prevented competence development, demonstrating the involvement of comS in this prokaryotic specialization process.
表面活性素合成酶基因座（srfA）中编码缬氨酸激活结构域的部分（srfA-d4）不仅参与了表面活性素的非核糖体合成，而且对于感受态发育的调控也是必需的。在本研究中，我们发现缬氨酸激活结构域的腺苷化活性受损并不影响感受态发育。缺失分析和互补研究将srfA-d4中感受态所需的部分定位到一个168 bp的片段，该片段包含一个小的开放阅读框（ORF），命名为comS，编码一个46个氨基酸的多肽，其嵌入srfA-d4的框架内，但翻译框架与srfA-d4不同。在comS编码框架中引入琥珀突变会阻止感受态发育，这表明comS参与了这一原核生物特化过程。
