枯草芽孢杆菌中包含感受态建立所需区域的srfA基因5'端部分的核苷酸序列。
Nucleotide sequence of 5' portion of srfA that contains the region required for competence establishment in Bacillus subtilus.
作者信息
Fuma S, Fujishima Y, Corbell N, D'Souza C, Nakano M M, Zuber P, Yamane K
机构信息
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
出版信息
Nucleic Acids Res. 1993 Jan 11;21(1):93-7. doi: 10.1093/nar/21.1.93.
Abstract
The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region, the first open reading frame, srfAA, encoding surfactin synthetase I and part of the second open reading frame, srfAB, encoding surfactin synthetase II. Three amino acid-activating domains characteristic of those found in peptide synthetases could be discerned in both srfAA (activating Glu, Leu and D-Leu) and srfAB (activating Val, Asp, and D-Leu). The presence of a conserved spacer motif in the amino-terminal end of srfAA suggests that the srfAA product may not initiate surfactin synthesis. The portion of srfA that contains the region required for competence is composed of srfAA and the first amino acid-activating domain of srfAB.
摘要
测定了srfA操纵子5'端20535个碱基对的核苷酸序列，其中包含感受态发育所需的区域。这包括srfA启动子区域、第一个开放阅读框srfAA（编码表面活性素合成酶I）和第二个开放阅读框srfAB的一部分（编码表面活性素合成酶II）。在srfAA（激活Glu、Leu和D-Leu）和srfAB（激活Val、Asp和D-Leu）中都能识别出肽合成酶中特有的三个氨基酸激活结构域。srfAA氨基末端存在保守的间隔基序，这表明srfAA产物可能不会启动表面活性素的合成。srfA中包含感受态所需区域的部分由srfAA和srfAB的第一个氨基酸激活结构域组成。
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