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Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae.在肠杆菌科革兰氏阴性菌中检测XerC和XerD重组酶。
J Bacteriol. 1995 Jul;177(14):4183-6. doi: 10.1128/jb.177.14.4183-4186.1995.
2
Cloning and characterisation of the Proteus mirabilis xerD gene.奇异变形杆菌xerD基因的克隆与鉴定
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Interactions of the Caulobacter crescentus XerC and XerD recombinases with the E. coli dif site.新月柄杆菌XerC和XerD重组酶与大肠杆菌dif位点的相互作用。
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Xer recombination in Escherichia coli. Site-specific DNA topoisomerase activity of the XerC and XerD recombinases.大肠杆菌中的Xer重组。XerC和XerD重组酶的位点特异性DNA拓扑异构酶活性。
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Interactions of the site-specific recombinases XerC and XerD with the recombination site dif.位点特异性重组酶XerC和XerD与重组位点dif的相互作用。
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C-terminal interactions between the XerC and XerD site-specific recombinases.XerC和XerD位点特异性重组酶之间的C端相互作用。
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Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12.在大肠杆菌K12中,dif和cer位点特异性重组需要两种相关的重组酶。
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Functional analysis of the C-terminal domains of the site-specific recombinases XerC and XerD.位点特异性重组酶XerC和XerD的C末端结构域的功能分析
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J Mol Biol. 1997 Jan 10;265(1):30-9. doi: 10.1006/jmbi.1996.0709.

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XerCD-mediated site-specific recombination leads to loss of the 57-kilobase gonococcal genetic island.XerCD 介导的位点特异性重组导致 57 千碱基淋病奈瑟氏菌遗传岛的缺失。
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本文引用的文献

1
Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12.在大肠杆菌K12中,dif和cer位点特异性重组需要两种相关的重组酶。
Cell. 1993 Oct 22;75(2):351-61. doi: 10.1016/0092-8674(93)80076-q.
2
Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif.质粒pSC101含有一个重组位点psi,它能够拆分质粒多聚体并替代大肠杆菌染色体上类似的位点dif。
J Bacteriol. 1994 Jun;176(11):3188-95. doi: 10.1128/jb.176.11.3188-3195.1994.
3
Xer-mediated site-specific recombination at cer generates Holliday junctions in vivo.Xer介导的cer位点特异性重组在体内产生霍利迪连接体。
EMBO J. 1994 Apr 15;13(8):1844-55. doi: 10.1002/j.1460-2075.1994.tb06453.x.
4
Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.高拷贝数质粒的多聚化会导致不稳定性:ColE1编码一个对质粒单体化和稳定性至关重要的决定因素。
Cell. 1984 Apr;36(4):1097-103. doi: 10.1016/0092-8674(84)90060-6.
5
Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.多拷贝质粒Clo DF13在大肠杆菌细胞中的维持:parB位点特异性重组的证据。
Cell. 1984 Jan;36(1):203-9. doi: 10.1016/0092-8674(84)90090-4.
6
Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site.ColE1 二聚体的解离需要一段与 cer 位点三维结构相关的 DNA 序列。
EMBO J. 1988 Mar;7(3):851-8. doi: 10.1002/j.1460-2075.1988.tb02884.x.
7
Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination.大肠杆菌中多拷贝质粒的稳定性需要宿主编码的功能,这些功能会导致质粒位点特异性重组。
Mol Gen Genet. 1988 Sep;214(1):80-4. doi: 10.1007/BF00340183.
8
Multimer resolution systems of ColE1 and ColK: localisation of the crossover site.大肠杆菌素E1和大肠杆菌素K的多聚体解析系统:交叉位点的定位
Mol Gen Genet. 1985;201(2):334-8. doi: 10.1007/BF00425680.
9
xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.xerB是大肠杆菌质粒ColE1位点特异性重组所需的基因,它与pepA相同,pepA编码氨肽酶A,该蛋白与牛晶状体亮氨酸氨肽酶有高度相似性。
EMBO J. 1989 May;8(5):1623-7. doi: 10.1002/j.1460-2075.1989.tb03547.x.
10
Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases.ColE1 cer位点的重组需要大肠杆菌xerC基因产物,它是位点特异性重组酶的λ整合酶家族的成员。
J Bacteriol. 1990 Dec;172(12):6973-80. doi: 10.1128/jb.172.12.6973-6980.1990.

在肠杆菌科革兰氏阴性菌中检测XerC和XerD重组酶。

Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae.

作者信息

Sirois S, Szatmari G

机构信息

Département de Microbiologie et Immunologie, Université de Montréal, Québec, Canada.

出版信息

J Bacteriol. 1995 Jul;177(14):4183-6. doi: 10.1128/jb.177.14.4183-4186.1995.

DOI:10.1128/jb.177.14.4183-4186.1995
PMID:7608100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177159/
Abstract

XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to cloned E. coli xerC and xerD genes and a cer-nmr plasmid-based resolution assay, the presence of these genes in several species of Enterobacteriaceae is shown.

摘要

XerC和XerD是λ整合酶家族的位点特异性重组酶,它们通过作用于特定位点(如质粒中发现的cer、ckr、nmr、parB和psi,或大肠杆菌染色体中发现的dif位点)将多聚体复制子分解为单体。通过对克隆的大肠杆菌xerC和xerD基因进行Southern杂交以及基于cer-nmr质粒的分解分析,表明这些基因存在于几种肠杆菌科细菌中。