在肠杆菌科革兰氏阴性菌中检测XerC和XerD重组酶。
Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae.
作者信息
Sirois S, Szatmari G
机构信息
Département de Microbiologie et Immunologie, Université de Montréal, Québec, Canada.
出版信息
J Bacteriol. 1995 Jul;177(14):4183-6. doi: 10.1128/jb.177.14.4183-4186.1995.
Abstract
XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to cloned E. coli xerC and xerD genes and a cer-nmr plasmid-based resolution assay, the presence of these genes in several species of Enterobacteriaceae is shown.
摘要
XerC和XerD是λ整合酶家族的位点特异性重组酶，它们通过作用于特定位点（如质粒中发现的cer、ckr、nmr、parB和psi，或大肠杆菌染色体中发现的dif位点）将多聚体复制子分解为单体。通过对克隆的大肠杆菌xerC和xerD基因进行Southern杂交以及基于cer-nmr质粒的分解分析，表明这些基因存在于几种肠杆菌科细菌中。
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本文引用的文献
1
Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12.在大肠杆菌K12中，dif和cer位点特异性重组需要两种相关的重组酶。
Cell. 1993 Oct 22;75(2):351-61. doi: 10.1016/0092-8674(93)80076-q.
2
Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif.质粒pSC101含有一个重组位点psi，它能够拆分质粒多聚体并替代大肠杆菌染色体上类似的位点dif。
J Bacteriol. 1994 Jun;176(11):3188-95. doi: 10.1128/jb.176.11.3188-3195.1994.
3
Xer-mediated site-specific recombination at cer generates Holliday junctions in vivo.Xer介导的cer位点特异性重组在体内产生霍利迪连接体。
EMBO J. 1994 Apr 15;13(8):1844-55. doi: 10.1002/j.1460-2075.1994.tb06453.x.
4
Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.高拷贝数质粒的多聚化会导致不稳定性：ColE1编码一个对质粒单体化和稳定性至关重要的决定因素。
Cell. 1984 Apr;36(4):1097-103. doi: 10.1016/0092-8674(84)90060-6.
5
Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.多拷贝质粒Clo DF13在大肠杆菌细胞中的维持：parB位点特异性重组的证据。
Cell. 1984 Jan;36(1):203-9. doi: 10.1016/0092-8674(84)90090-4.
6
Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site.ColE1 二聚体的解离需要一段与 cer 位点三维结构相关的 DNA 序列。
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