Elaeva N L, Stroĭkov Iu N
Biull Eksp Biol Med. 1979 Jan;87(1):24-7.
Eserine (1-2 mg/kg) decreased incorporation of the radioactive precursors into total DNA of the rat liver by 40-50%. Only high eserine cincentrations (10-5 M) effected directly the DNA synthesis in the hepatocyte nuclei. It was shown in vitro that the DNA synthesis in the nuclei of hepatocytes, isolated from the liver of rats after eserine administration (1 mg/kg), decreased significantly. A conclusion is drawn that the eserine action on the incorporation of radioactive precursors into DNA of the rat liver in vivo is localized at the level of the DNA synthesis in the nuclei. The study of the acetylcholine action on the DNA synthesis in the isolated nuclei of hepatocytes suggests that the inhibitory effect of eserine on the DNA synthesis is mediated through acetylcholine.
毒扁豆碱（1 - 2毫克/千克）使放射性前体掺入大鼠肝脏总DNA的量减少了40 - 50%。只有高浓度的毒扁豆碱（10 - 5摩尔）直接影响肝细胞细胞核中的DNA合成。体外实验表明，给大鼠注射毒扁豆碱（1毫克/千克）后，从其肝脏分离出的肝细胞细胞核中的DNA合成显著减少。得出的结论是，毒扁豆碱在体内对放射性前体掺入大鼠肝脏DNA的作用定位于细胞核中DNA合成水平。对乙酰胆碱对分离的肝细胞细胞核中DNA合成作用的研究表明，毒扁豆碱对DNA合成的抑制作用是通过乙酰胆碱介导的。
